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This research was conducted to study the dengue situation and environmental factors and Ecology in different sub-districts and environments in Chiang Mai Province. The objectives of the study are to gather various dengue situations, survey environmental factors impacting on ecology and factors of mosquitoes carrying dengue disease, by evaluating the mosquito larvae index in Chom Thong District, Hang Dong District and Mueang District, Chiang Mai Province. Subsequently, the information was analyzed and interpreted, and the results of the study were used as a guideline for prevention and control of dengue fever by using Montfort College School as a research base. The collected data from 98 different study sites, comprising 38 houses in Chom Thong District, 40 houses in Hang Dong District, and 20 houses in Mueang District during November 2018 to January 2019. Data analysis was made by using frequency distribution, percentage, mean and calculation of mosquito larvae index. The study indicated that the differences of community areas affect dengue fever situations and the mosquito larvae index in 3 study sites are different. Due to the characteristics of the communities in which the study site took place, it was found that different distribution characteristics and different ecological environmental factors affect the mosquito larvae index. The study indicated that the most common mosquito larvae are found in a man-made container. However, containers inside the house in the city have not been explored more thoroughly because of restrictions on the survey. For instance, the landlord of some properties went to work, making it possible to explore the outside of the house only.