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This research aimed to evaluate the effectiveness of ethanolic extracts of Tinospora crispa (boraphet vine) in eliminating Aedes mosquito larvae and to examine the relationship between extract concentration and larval mortality under controlled conditions. The experimental design was based on three extract concentrations: 2.5, 5, and 10 ppm, and three different test volumes: 10, 20, and 30 milliliters. Each concentration was compared with a control group using clean water. All experiments were conducted in three replicates. Environmental factors such as temperature, light intensity, and pH were carefully controlled to ensure suitable conditions for larval survival. Larval mortality and physical changes were recorded continuously over a period of 7 days. The results showed that the boraphet extract caused a statistically significant increase in larval mortality (P < 0.05). The mortality rate was positively correlated with extract concentration, indicating that higher concentrations resulted in higher larval death rates. When the concentration increased from 2.5 ppm to 10 ppm, larval mortality increased accordingly. In addition, observable physical changes were found in larvae exposed to higher extract concentrations, such as reduced movement and immobility prior to death. In conclusion, the experiment demonstrated that boraphet extract contains bioactive compounds with strong potential to inhibit growth and effectively eliminate mosquito larvae. Therefore, it can be considered a natural alternative for controlling mosquito breeding sites and preventing the spread of dengue fever, reducing reliance on synthetic chemical larvicides while being safer for humans and environmentally friendly in community settings. Keywords: Tinospora crispa (Boraphet), Aedes mosquito larvae, herbal extract, insect control, dengue fever