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This research aimed to investsate the prevalence of Aedes mosquito larvae and its relationship with weather factors affecting the occurrence of dengue fever in Phak Mai Subdistrict, Huai Thap Than District, Sisaket Province. The survey of Aedes mosquito larvae was conducted in households and water storage containers in three villages: Ban Du, Ban Phrai Phanaow, and Ban Ta Thong, from June to August 2025. Data collection was carried out through field surveys, and larval prevalence was analyzed using standard indices, namely the House Index (HI) and Breteau Index (BI), together with weather data including rainfall, relative humidity, and temperature obtained from the Sisaket Provincial Meteorological Station. The results showed that the overall House Index and Breteau Index values were at levels indicating a risk of dengue fever transmission. All villages had HI values greater than 1 0 and BI values consistently above the standard threshold, especially during periods of high cumulative rainfall and high relative humidity, which favor the formation of breeding sites and the reproduction of Aedes mosquitoes. In contrast, villages with proper management of water storage containers exhibited lower larval indices than those with inadequate management. The findings indicate that changes in weather conditions, particularly rainfall and relative humidity, are associated with the prevalence of Aedes mosquito larvae and the risk of dengue fever in the community. The information obtained from this study can be used as a guideline for surveillance, planning, prevention, and effective control of dengue fever at the community level. Keywords: Aedes mosquito larvae, Weather conditions, Larval indices (HI, BI), Dengue fever