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This research aimed to investigate the quality of drinking water from natural water sources in Ban Mae Tao Din and Ban Huai Kaeo, Huai Kaeo Subdistrict, Mae On District, Chiang Mai Province. These water sources are used by the local community for domestic consumption, daily use, and agricultural purposes. The study evaluated the physical and chemical properties of the water and compared the results with the Thailand Drinking Water Quality Standards and the World Health Organization (WHO) guidelines. Water samples were collected from three sources: groundwater from Ban Huai Kaeo, groundwater from Ban Mae Tao Din, and mountain runoff water in the Ban Mae Tao Din area (upstream, midstream, and downstream). Sampling was conducted during three periods between 2 August 2025 and 18 January 2026, covering both the rainy and winter seasons. Water quality parameters analyzed included pH, Total Dissolved Solids (TDS), and contamination by ammonia and nitrate, using standard water quality testing instruments. The data were analyzed using descriptive statistics. The results showed that water from all sources was clear, odorless, and free from abnormal coloration. The pH values were within acceptable standard ranges, TDS values were below the permissible limits, and no ammonia or nitrate contamination was detected during any sampling period. These findings indicate that the natural water sources in the study area are of acceptable quality and suitable for domestic consumption. The results can serve as baseline information for community-based water resource management and conservation, contributing to sustainable development goals (SDGs) related to public health and environmental sustainability.