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Water quality degradation from human activities is a growing concern for local communities. This study evaluates the water quality and macroinvertebrate diversity in two vital water resources: Nong Plong and Huai Wa in Sisaket Province. Residents rely on these sites for agriculture, consumption, and local fishing. By monitoring physical and chemical parameters—temperature, pH, Dissolved Oxygen (DO), and transparency—alongside biological surveys, we assessed the health of these ecosystems. Our findings revealed that Nong Plong maintains better water quality, characterized by higher DO levels and the presence of sensitive species like dragonfly larvae. In contrast, Huai Wa showed signs of organic accumulation indicated by the presence of freshwater leeches. This research confirms that macroinvertebrates serve as effective natural medicine for monitoring environmental health. Keywords Chemical and Physical Parameters ,Macroinvertebrates.