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From the study, it was found that in the area of Na Yong District, 4 types of mosquito larvae were found, namely Aedes albopictus larvae, followed by Culex larvae , Aedes aegypti larvae and Toxorhynchites spp. , respectively. In Palian district, 2 types of mosquito larvae were found, namely the most common Aedes aegypti larvae, followed by the Aedes albopictus larvae , the mosquito larva breeding sites for mosquito larvae were found. In Na Yong District, there are 7 sources, namely, coconut shells, rubber trays, broken plastic containers , tires, water tanks, water pipes (mortar) and waste bins. In Palian District, there are 4 breeding sites for mosquito larvae, namely water buckets, large water tanks, coconut shells and tires. The pH water quality values according to various sources of mosquito larvae in Na Yong compared to Palian district, the pH values in Na Yong and Palian districts have no significant difference. The average surface water temperature of the mosquito larvae habitat in Palian was higher than in Na Yong district and water temperature of mosquito larvae habitats in Na Yong District was higher than that in Palian District. In Palian, the Aedes albopictus and Aedes aegypti larval HI and CI values were higher than in Na Yong district, while BI values in Na Yong were higher than in Palian district. From the study of species and number of mosquito larvae in Na Yong and Palian district, it was found that, in Na Yong district, the most common Aedes albopictus larvae were found. In Palian district, the most common Aedes aegypti larvae were found, which is classified as a moderate risk level to cause dengue, yellow fever and possibly a carrier of the Zika virus. The communities of both areas have been identified as having problems spreading at some point.