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This study aims to examine the changes in water before and after adding artificial watercolor in terms of physical and chemical properties, as well as differences in living organisms in the water source between Building 2 and Building 3 at Wichienmatu School, Khok Lor Subdistrict, Mueang Trang District, Trang Province. The water samples were collected between November 20, 2024, and January 22, 2025. The samples were analyzed for cloudiness, iron content, phosphate content, nitrate content, dissolved oxygen, temperature, electrical conductivity, pH, and water transparency. Before adding artificial watercolor, it was found that the cloudiness level was 70%, the electrical conductivity was 0.2 ppt, the water transparency was 68.5 cm, the temperature was 33°C, the pH level was 8.1, the dissolved oxygen concentration was 5.5 ml/L, the iron content was 0.1 mg/L, and the phosphate content was 0.1 mg/L. After adding artificial watercolor, it was found that the cloudiness level was 67.81%, the iron content remained at 0.1 mg/L, and the phosphate content remained at 0.1 mg/L. The dissolved oxygen concentration ranged from 4.5 to 6 ml/L, the water temperature ranged from 26 to 29°C, the electrical conductivity ranged from 0.1 to 0.2 ms, the pH level ranged from 7.7 to 8.86, and the water transparency ranged from 49.3 to 86.0 cm. Nitrate content was not detected in the water source. After adding artificial watercolor, the number of small organisms decreased, while the number of large organisms increased